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human mammary fibroblasts primary cell (hmfpc)  (Procell Inc)

 
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    Procell Inc human mammary fibroblasts primary cell (hmfpc)
    SAPCD2 is upregulated in fibrosarcoma tissues and cells. (A) Real-time PCR and Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung <t>fibroblasts</t> primary cell <t>(hLFPC),</t> human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. GAPDH was used as endogenous controls in RT-PCR and α-Tubulin was detected as a loading control in the Western blot. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. (B) Representative images of SAPCD2 expression in benign fibroma, dermatofibrosarcoma protuberans (DFSP), and fibrosarcoma tissues. (C) The number of fibroma, DFSP, and fibrosarcoma tissues stratified by staining index of IHC. (D) Immunohistochemical staining index of SAPCD2 in fibroma, DFSP, and fibrosarcoma tissues. n.s. indicates no significance.
    Human Mammary Fibroblasts Primary Cell (Hmfpc), supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mammary+fibroblasts+primary+cell+(hmfpc)/human+mammary+fibroblasts+primary+cell++hmfpc+/pmc07770171-92-10-35
    Average 90 stars, based on 1 article reviews
    human mammary fibroblasts primary cell (hmfpc) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway"

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2020.574383

    SAPCD2 is upregulated in fibrosarcoma tissues and cells. (A) Real-time PCR and Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. GAPDH was used as endogenous controls in RT-PCR and α-Tubulin was detected as a loading control in the Western blot. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. (B) Representative images of SAPCD2 expression in benign fibroma, dermatofibrosarcoma protuberans (DFSP), and fibrosarcoma tissues. (C) The number of fibroma, DFSP, and fibrosarcoma tissues stratified by staining index of IHC. (D) Immunohistochemical staining index of SAPCD2 in fibroma, DFSP, and fibrosarcoma tissues. n.s. indicates no significance.
    Figure Legend Snippet: SAPCD2 is upregulated in fibrosarcoma tissues and cells. (A) Real-time PCR and Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. GAPDH was used as endogenous controls in RT-PCR and α-Tubulin was detected as a loading control in the Western blot. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. (B) Representative images of SAPCD2 expression in benign fibroma, dermatofibrosarcoma protuberans (DFSP), and fibrosarcoma tissues. (C) The number of fibroma, DFSP, and fibrosarcoma tissues stratified by staining index of IHC. (D) Immunohistochemical staining index of SAPCD2 in fibroma, DFSP, and fibrosarcoma tissues. n.s. indicates no significance.

    Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Staining, Immunohistochemical staining

    SAPCD2 inactivates Hippo signaling pathway. (A) GSEA analysis showed that SAPCD2 expression level was associated with the Hippo signaling. (B) TEAD transcriptional activity was assessed by HOP-Flash luciferase reporter in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (C) Western blot analysis of total p-MST1/2, MST1, p-LATS1, LATS1, p-YAP1, YAP1 expression and nuclear YAP, TAZ expression in the indicated cells. α-Tubulin was detected as a loading control and nuclear protein p84 was used as the nuclear protein marker. (D) Real-time PCR analysis of CTGF, CYR61, SOX9, HOXA1, RPL13A, and PPIA in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (E) Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. α-Tubulin was detected as a loading control.
    Figure Legend Snippet: SAPCD2 inactivates Hippo signaling pathway. (A) GSEA analysis showed that SAPCD2 expression level was associated with the Hippo signaling. (B) TEAD transcriptional activity was assessed by HOP-Flash luciferase reporter in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (C) Western blot analysis of total p-MST1/2, MST1, p-LATS1, LATS1, p-YAP1, YAP1 expression and nuclear YAP, TAZ expression in the indicated cells. α-Tubulin was detected as a loading control and nuclear protein p84 was used as the nuclear protein marker. (D) Real-time PCR analysis of CTGF, CYR61, SOX9, HOXA1, RPL13A, and PPIA in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (E) Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. α-Tubulin was detected as a loading control.

    Techniques Used: Expressing, Activity Assay, Luciferase, Western Blot, Control, Marker, Real-time Polymerase Chain Reaction

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Western Blot:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Expressing:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Control:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Staining:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Immunohistochemical staining:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Activity Assay:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Luciferase:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A

    Marker:

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway
    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). .. A



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    human mammary fibroblasts primary cell (hmfpc)  (Procell Inc)
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    Procell Inc human mammary fibroblasts primary cell (hmfpc)
    SAPCD2 is upregulated in fibrosarcoma tissues and cells. (A) Real-time PCR and Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung <t>fibroblasts</t> primary cell <t>(hLFPC),</t> human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. GAPDH was used as endogenous controls in RT-PCR and α-Tubulin was detected as a loading control in the Western blot. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. (B) Representative images of SAPCD2 expression in benign fibroma, dermatofibrosarcoma protuberans (DFSP), and fibrosarcoma tissues. (C) The number of fibroma, DFSP, and fibrosarcoma tissues stratified by staining index of IHC. (D) Immunohistochemical staining index of SAPCD2 in fibroma, DFSP, and fibrosarcoma tissues. n.s. indicates no significance.
    Human Mammary Fibroblasts Primary Cell (Hmfpc), supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mammary+fibroblasts+primary+cell+(hmfpc)/human+mammary+fibroblasts+primary+cell++hmfpc+/pmc07770171-92-10-35
    Average 90 stars, based on 1 article reviews
    human mammary fibroblasts primary cell (hmfpc) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    SAPCD2 is upregulated in fibrosarcoma tissues and cells. (A) Real-time PCR and Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. GAPDH was used as endogenous controls in RT-PCR and α-Tubulin was detected as a loading control in the Western blot. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. (B) Representative images of SAPCD2 expression in benign fibroma, dermatofibrosarcoma protuberans (DFSP), and fibrosarcoma tissues. (C) The number of fibroma, DFSP, and fibrosarcoma tissues stratified by staining index of IHC. (D) Immunohistochemical staining index of SAPCD2 in fibroma, DFSP, and fibrosarcoma tissues. n.s. indicates no significance.

    Journal: Frontiers in Oncology

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway

    doi: 10.3389/fonc.2020.574383

    Figure Lengend Snippet: SAPCD2 is upregulated in fibrosarcoma tissues and cells. (A) Real-time PCR and Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. GAPDH was used as endogenous controls in RT-PCR and α-Tubulin was detected as a loading control in the Western blot. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. (B) Representative images of SAPCD2 expression in benign fibroma, dermatofibrosarcoma protuberans (DFSP), and fibrosarcoma tissues. (C) The number of fibroma, DFSP, and fibrosarcoma tissues stratified by staining index of IHC. (D) Immunohistochemical staining index of SAPCD2 in fibroma, DFSP, and fibrosarcoma tissues. n.s. indicates no significance.

    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China).

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Staining, Immunohistochemical staining

    SAPCD2 inactivates Hippo signaling pathway. (A) GSEA analysis showed that SAPCD2 expression level was associated with the Hippo signaling. (B) TEAD transcriptional activity was assessed by HOP-Flash luciferase reporter in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (C) Western blot analysis of total p-MST1/2, MST1, p-LATS1, LATS1, p-YAP1, YAP1 expression and nuclear YAP, TAZ expression in the indicated cells. α-Tubulin was detected as a loading control and nuclear protein p84 was used as the nuclear protein marker. (D) Real-time PCR analysis of CTGF, CYR61, SOX9, HOXA1, RPL13A, and PPIA in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (E) Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. α-Tubulin was detected as a loading control.

    Journal: Frontiers in Oncology

    Article Title: Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway

    doi: 10.3389/fonc.2020.574383

    Figure Lengend Snippet: SAPCD2 inactivates Hippo signaling pathway. (A) GSEA analysis showed that SAPCD2 expression level was associated with the Hippo signaling. (B) TEAD transcriptional activity was assessed by HOP-Flash luciferase reporter in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (C) Western blot analysis of total p-MST1/2, MST1, p-LATS1, LATS1, p-YAP1, YAP1 expression and nuclear YAP, TAZ expression in the indicated cells. α-Tubulin was detected as a loading control and nuclear protein p84 was used as the nuclear protein marker. (D) Real-time PCR analysis of CTGF, CYR61, SOX9, HOXA1, RPL13A, and PPIA in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. * P < 0.05 . (E) Western blotting analysis of SAPCD2 expression in 4 normal fibroblast cell lines, including human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), Human embryonic lung fibroblast (HFL1), and 2 fibrosarcoma cell lines HT-1080 and SW684. α-Tubulin was detected as a loading control.

    Article Snippet: The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China).

    Techniques: Expressing, Activity Assay, Luciferase, Western Blot, Control, Marker, Real-time Polymerase Chain Reaction